Validate Your Oligo Pool Before Vendor Upload
Run sequence QC, check synthesis risk, and export vendor-ready files before you submit CRISPR libraries, NGS panels, gene assembly pools, or mutagenesis designs for synthesis.
Page role: this homepage is the calculator-led workflow router for OligoPool. Use it to choose the next stable-attractor workspace for batch QC, synthesis-risk calculation, coverage sizing, vendor-format export, or oligo-pool planning.
The Oligo Pool Processing Pipeline
Follow our standardized 4-step workflow to validate your oligonucleotide pool design before synthesis. Ensure high uniformity and prevent sequence dropout.
Format Converter
Transform sequences between FASTA and CSV with strict validation rules.
Batch Sequence QC
Concurrent screening of up to 10,000 sequences for Tm, GC limits, and secondary structures.
Error Rate & Uniformity
Forecast the full-length percentage, coupling efficiency effects, and pool uniformity distribution.
Coverage Calculator
Determine the exact synthesis scale required to prevent sequence dropout and ensure full representation.
Built for High-Throughput Applications
Explore application-specific workflows for CRISPR, NGS, and multiplexed oligo pool design.
CRISPR sgRNA Libraries
Guidelines and workflow for designing sgRNA libraries with high on-target activity and minimum off-target effects.
NGS Target Enrichment
Design hybridization capture probes with balanced GC content for uniform enrichment.
Multiplex PCR Panels
Mitigate primer-dimer formation and Tm imbalances to ensure even amplification across hundreds of targets.
Why Use These Tools
Scientifically Accurate
Based on peer-reviewed methods (SantaLucia 1998, Breslauer 1986). All formulas and assumptions are transparent and documented.
Privacy Focused
All calculations run in your browser. Sequences and result rows are not transmitted to our servers; analytics only records aggregate site interactions.
100% Free
No registration required, no paywalls, no vendor bias. Built as a personal project for the research community.
Vendor-Agnostic Design Processing
Move from a checked sequence pool to a vendor decision path and export format. Compare pool fit, QC scope, delivery assumptions, and then adapt files for the selected ordering workflow.
Start with the Oligo Pool WorkflowCompare pool vendorsHow We Compare
Head-to-head comparisons with the most popular oligo analysis tools.
Ready to Start Designing?
Browse all available tools or jump straight to the most popular calculator. No registration required, start calculating immediately. Need help? Check out our use case examples for step-by-step workflows.
Trusted by researchers worldwide
What is an Oligo Pool?
An oligo pool is a complex mixture of thousands to millions of distinct single-stranded DNA oligonucleotides synthesized in parallel on a microarray surface. Unlike traditional column-based synthesis which produces individual oligos in separate tubes, microarray synthesis generates massive libraries of sequences simultaneously in a single pool.
This fundamental shift in synthesis technology has revolutionized functional genomics. Oligo pools are the essential building blocks for high-throughput applications such as designing CRISPR sgRNA pools for pooled screening, assembling synthetic genes from oligo pools, and building custom NGS target-enrichment panels.
Due to the inherent constraints of parallel synthesis, such as varying coupling efficiencies and edge effects on the microarray, computational quality control is critical. Predicting error rates and ensuring uniform sequence representation before ordering your pool can prevent costly experimental failures downstream.