For Biotech Companies
Biotech Oligo Design Tools for CRISPR, QC and Orders
Browser-based tools for CRISPR library design, oligo pool QC, and vendor order preparation. Use them for early review, ordering checks, and team review files with browser-side calculations by default; optional saves transmit only selected summaries or explicit Library assets.
Use this page to connect project tasks with the right calculator: batch QC, coverage sizing, vendor-format export, GC balance analysis, structure checks, synthesis-risk modeling, and dilution planning.
Why Biotech Teams Use OligoPool
No IT Overhead
Browser-based tools require zero installation, no IT tickets, no dependency conflicts. Works on any laptop, any OS. New team members productive in seconds, not days.
Data Security by Design
Calculations run client-side in JavaScript by default. Optional Dashboard saves transmit only selected summaries or explicitly saved sequence assets, so teams can decide what becomes a reusable backend record.
Scale Without Licensing
Process 1,000 sequences free or 50,000 sequences with Tool Membership per batch analysis. Your team can use the browser tools without per-seat setup.
Review-Ready Outputs
Export results as CSV and generate vendor-specific order files for IDT, Twist, GenScript, and other ordering paths.
Biotech Project Tasks
Tools organized by the sequence review stage.
CRISPR Screen Library Design & Validation
Design and validate genome-scale sgRNA libraries before synthesis. Ensure every guide is synthesis-compatible, free of problematic structures, and meets uniformity requirements for powered screens.
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Review value
Catch synthesis-incompatible guides before ordering. Reduce redesign cycles from 3 rounds to 1.
Targeted Panel & Capture Probe Design
Design hybridization capture probes or targeted sequencing panels. Optimize Tm uniformity across all probes for even capture efficiency.
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Review value
Achieve uniform capture efficiency across your panel by identifying and adjusting Tm outliers.
Gene Synthesis & Assembly
Design overlapping oligos for Gibson assembly or gene synthesis. Verify overlap Tm, check for mispriming, and prepare vendor orders.
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Review value
Predict synthesis quality before ordering and plan appropriate purification strategies.
Vendor Integration
Generate order-ready files for your preferred synthesis vendor.
IDT oPools
Excel/CSV export ready
Twist Bioscience
Excel/CSV export ready
GenScript
Excel/CSV export ready
Dynegene
Excel/CSV export ready
Accelerate Oligo Design and Review
Free tools your entire team can use today. No procurement process needed.