Oligo Vendor Format Converter - IDT, Twist, GenScript & Dynegene

Convert FASTA, CSV, or plain text sequences to vendor-specific order formats for IDT oPools,Twist Bioscience, GenScript, and Dynegene. Automatically validate sequences, calculate GC content, and export Excel/CSV files ready for direct upload to vendor ordering systems. Updated for 2026 vendor specifications.

Batch processing: 10,000 sequencesClient-side: Complete data privacyFree tool: No registration required

Input Sequences

You have chosen IDT format. Next, check sequence names, length limits, and oPools-specific columns before export.

Vendor selected
Sequences loaded
Options set
Convert and export

Accepts FASTA, CSV, or plain sequences. Max 10,000 sequences.

Naming Options

Enter sequences to see results

Converted data will appear here

Why You Need a Vendor Format Adapter

Each oligonucleotide synthesis vendor has its own proprietary ordering format — different column names, required fields, scale options, purification codes, and file formats. Converting your sequences from a universal format (FASTA, CSV) into vendor-specific order files is tedious and error-prone when done manually, especially for large oligo pools with thousands of sequences.

The Vendor Format Adapter supports IDT, Twist Bioscience, GenScript, and Dynegene-style export templates. It generates Excel/CSV files with the column structure, sequence names, length fields, GC% values, and selected order options needed for a cleaner submission handoff.

This page is the primary vendor order-format workspace on OligoPool. Use it when the search task is to convert sequences into vendor-specific order files; use the vendor directory, comparison, and ordering guide pages as support when you still need provider discovery, vendor selection, or submission context.

Use the adapter as a pre-submission formatting and review step, not as the final source of vendor capability truth. It flags sequence length, unsupported characters, and GC content concerns so you know what to verify in the current vendor portal, quote, or public product page before ordering.

How to Use the Vendor Format Adapter

  1. Upload your sequences: paste from clipboard, upload a FASTA/CSV file, or paste tab-separated name-sequence pairs.
  2. Select the target export template: IDT, Twist Bioscience, GenScript, or Dynegene.
  3. Configure order options: synthesis scale (25 nmol, 100 nmol, etc.), purification method (Desalting, HPLC, PAGE), and naming scheme.
  4. Review validation results: sequences with length, character, GC%, or formatting concerns are flagged for manual review.
  5. Download the formatted Excel or CSV file, then confirm the current vendor portal requirements before submission.

Frequently Asked Questions

What format does IDT require for oligo pool orders?
The IDT export template uses order-file fields such as pool or oligo name, sequence, scale, purification, and optional notes depending on the selected mode. Treat the exported file as a clean starting point: verify the current IDT portal columns, product tier, sequence-length window, and modification rules before submitting an order.
What are the sequence length limits for each vendor?
The adapter flags unusually short or long sequences so they can be reviewed before upload. Current limits depend on vendor, product tier, modification status, and quote terms, so confirm the final length window on the vendor portal or in the public-spec snapshot before ordering.
How do I choose between vendors for my oligo pool?
Use this page after you have selected a vendor format. For vendor choice, compare pool size, sequence length, QC scope, delivery amount, turnaround, and quote terms on the oligo pool vendor comparison page, then confirm the current public-spec snapshot before submitting files.
Can I include modifications in my vendor order?
You can include modification fields in export templates that support them, but modification availability depends on the vendor, product tier, sequence length, scale, and quote. Keep modifications explicit in the file and verify compatibility directly before ordering.
What happens if my sequence contains invalid characters?
Our adapter validates all sequences against the target vendor's accepted alphabet. Most vendors accept standard DNA bases (A, T, C, G) and some accept IUPAC ambiguity codes (R, Y, S, W, etc.) depending on the product type. Sequences containing invalid characters (numbers, spaces, non-IUPAC letters) are flagged with specific error messages. You can fix these in the tool before downloading the order file, preventing submission errors and order delays.

Related Tools

Format Converter

Convert between FASTA, CSV, TSV formats before using the Vendor Format Adapter.

Batch Sequence QC

Validate all sequences for synthesis compatibility before generating vendor order files.

Uniformity Estimator

Predict expected uniformity and dropout rate for your chosen synthesis platform.

Project Review

Check a Result or Ordering Step

Send the calculation, settings, or pool submission detail that needs a second look.

Next Pages to Open

Continue with the page or tool that matches the next decision in your experiment.

Oligo Pool Vendor ComparisonVendor Public-Spec SnapshotPre-Order Oligo Pool QCUser Guide