Wet-lab / NGS Validation

NGS Pool Representation QC Analyzer

Compare designed-library rows with read counts to flag dropout, zero-count oligos, Gini skew, CV, and fold representation.

Closed loop: clean input, design architecture, run pre-synthesis QC, validate representation, then export vendor/report files.

Privacy: calculations run in this browser; history stores only aggregate counts and summary metrics. Generated sequence assets save to the backend only when you click save.

Next: use the Oligo Pool Guide, Batch QC, Vendor Adapter, and QC templates after results.

Input

Start with the sample values, then replace them with your own design rows.

Designed library CSV

CSV columns: ID, Sequence.

Read-count CSV

CSV columns: ID, Count. FASTQ parsing is intentionally out of scope for this browser MVP.

Minimum reads per oligo

Dropout threshold (% of mean)

Results

Results will appear here with downloadable CSV/FASTA outputs where applicable.

Oligo Pool Guide

Use the main pool workflow to connect representation QC findings back to redesign, vendor, and ordering decisions.