Lab workflows
Oligo Design Tools by Lab Type
Choose the audience path that matches the work in front of you, then move into the calculator, sequence QC, vendor file, or oligo pool planning page that owns the actual task.
Choose an Audience Path
These pages organize common lab situations without replacing the calculator or oligo pool workflow pages.
Academic labs
Free primer, dilution, CRISPR coverage, and batch sequence QC workflows for research and teaching labs.
Biotech teams
Browser-side tools for CRISPR libraries, targeted panels, vendor files, and repeatable pre-order checks.
Pharma R&D
Sequence checks for target validation, qPCR assay work, ASO review, vendor management, and study documentation.
Open the Primary Workflow
Start with the page that produces the result or decision, then use the audience pages for context.
Start with the Oligo Pool workflowUse this route when the job includes pool design, synthesis choices, QC, vendors, and ordering decisions.
Calculate primer TmOpen the Tm Calculator for matched salt settings, batch primer checks, and method-based melting temperature results.
Run all-in-one primer reviewUse the Primer Analyzer for Tm, GC%, hairpin, dimer, mismatch, and BLAST handoff checks in one workspace.
Screen a sequence poolUse Batch Sequence QC to review 1,000 free or 50,000 member oligos for GC, Tm, length, homopolymers, and sequence risk.
Size CRISPR coverageOpen the Coverage Calculator for screen representation, tiling libraries, and custom oligo pool sizing.
Prepare vendor order filesUse Vendor Format Adapter when sequence data is ready and the order needs vendor-specific columns.
Prepare stocks and dilutionsUse the Dilution Calculator for resuspension volumes, working stocks, pool dilutions, and printable prep steps.