Design / Architecture

DMS / Saturation Mutagenesis Library Planner

Plan degenerate codon libraries, estimate variant count, stop-codon burden, and transformant coverage before oligo synthesis.

Closed loop: clean input, design architecture, run pre-synthesis QC, validate representation, then export vendor/report files.

Privacy: calculations run in this browser; history stores only aggregate counts and summary metrics. Generated sequence assets save to the backend only when you click save.

Next: use the Oligo Pool Guide, Batch QC, Vendor Adapter, and QC templates after results.

Input

Start with the sample values, then replace them with your own design rows.

Protein sequence

Target positions (1-based)

Allowed amino acids

Degenerate codon scheme

Custom codon

Used only when scheme is Custom.

Coverage probability (%)

Transformants per variant

Results

Results will appear here with downloadable CSV/FASTA outputs where applicable.

Oligo Pool Guide

Use the main pool workflow to connect mutagenesis diversity, construct design, QC, and vendor preparation.