Wet-lab / NGS Validation

CRISPR Library Experiment Planner

Estimate guide representation, MOI-adjusted seed cells, replicate scale, controls, and selection bottleneck before screening.

Closed loop: clean input, design architecture, run pre-synthesis QC, validate representation, then export vendor/report files.

Privacy: calculations run in this browser; history stores only aggregate counts and summary metrics. Generated sequence assets save to the backend only when you click save.

Next: use the Oligo Pool Guide, Batch QC, Vendor Adapter, and QC templates after results.

Input

Start with the sample values, then replace them with your own design rows.

Experimental guide count

Coverage per guide

MOI

Replicates

Pool uniformity fold

Control guide %

Selection bottleneck retained %

Results

Results will appear here with downloadable CSV/FASTA outputs where applicable.

Oligo Pool Guide

Use the main pool workflow to connect CRISPR screen scale, coverage, synthesis constraints, and QC decisions.